Penelobee
Queen Penelobee (Catherine Disher) is a human/alien bee-hybrid, made using the DNA of Penelope Lang. After the death of their previous queen, the alien bee people of Droneopolis decided to choose a new queen, scouring the galaxy to find the "most despicable female" in the cosmos, observing candidates such as Iciclia and Nuclea; in the end, they had concluded that this person was none other then Penelope. Penelope was abducted by the Bee People, but was later rescued by Betty and her crew; all the while, Penelope had thought the incident was only a dream. However, the bees of Droneopolis had managed to retain a strand of Penelope's hair. DNA from the hair was combined with some of their own genetic material to create a hybrid clone of Penelope to serve as their new queen.
Queen Penelobee is similar to Penelope in most aspects, but is unfamiliar with Betty (ironic as Betty and Penelope interact regularly) and has romantic feelings for Sparky, after he had posed as a "king bee" in "Bee Movie".